Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 90737..91262 Replicon plasmid pST95-32-2
Accession NZ_CP043952
Organism Escherichia coli strain ST95-32

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag F3J96_RS27065 Protein ID WP_001159871.1
Coordinates 90957..91262 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag F3J96_RS27060 Protein ID WP_000813630.1
Coordinates 90737..90955 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3J96_RS27025 86153..86569 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
F3J96_RS27030 86566..86796 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
F3J96_RS27035 87061..87561 + 501 WP_000528932.1 hypothetical protein -
F3J96_RS27040 87574..88347 + 774 WP_000905949.1 hypothetical protein -
F3J96_RS27045 88514..89647 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
F3J96_RS27050 89681..90193 - 513 WP_000151784.1 hypothetical protein -
F3J96_RS27060 90737..90955 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F3J96_RS27065 90957..91262 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
F3J96_RS27070 91263..92069 + 807 WP_000016968.1 site-specific integrase -
F3J96_RS27075 92749..93480 - 732 WP_000504262.1 replication initiation protein -
F3J96_RS27080 94101..95306 + 1206 WP_001442122.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..189994 189994


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T139666 WP_001159871.1 NZ_CP043952:90957-91262 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T139666 NZ_CP058771:3817349-3817627 [Shigella flexneri]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT139666 WP_000813630.1 NZ_CP043952:90737-90955 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT139666 NZ_CP058771:3817086-3817346 [Shigella flexneri]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References