Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 86153..86796 Replicon plasmid pST95-32-2
Accession NZ_CP043952
Organism Escherichia coli strain ST95-32

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag F3J96_RS27025 Protein ID WP_001034046.1
Coordinates 86153..86569 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag F3J96_RS27030 Protein ID WP_001261278.1
Coordinates 86566..86796 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3J96_RS27010 81290..81706 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
F3J96_RS27015 81703..81933 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
F3J96_RS27020 82314..86108 + 3795 WP_190319101.1 pcar -
F3J96_RS27025 86153..86569 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
F3J96_RS27030 86566..86796 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
F3J96_RS27035 87061..87561 + 501 WP_000528932.1 hypothetical protein -
F3J96_RS27040 87574..88347 + 774 WP_000905949.1 hypothetical protein -
F3J96_RS27045 88514..89647 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
F3J96_RS27050 89681..90193 - 513 WP_000151784.1 hypothetical protein -
F3J96_RS27060 90737..90955 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
F3J96_RS27065 90957..91262 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..189994 189994


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T139665 WP_001034046.1 NZ_CP043952:c86569-86153 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T139665 NZ_CP058771:3632601-3632999 [Shigella flexneri]
GTGCGTAATAGCGACTGTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGC
ATATTCGCTGGAAAAACAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCA
TGTTAGGGGTAGCGCGGAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTAATTTTTTTGAACATGTAAAAATA
ATTCACCGGGCATTACCAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTT
TGTTCAGCTTTCAGCGAGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT139665 WP_001261278.1 NZ_CP043952:c86796-86566 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT139665 NZ_CP058771:3632163-3632474 [Shigella flexneri]
ATGCACGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References