Detailed information of TA system TA139663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 14581..14957
• Replicon: plasmid pST95-32-2
• Accession: NZ_CP043952
• Organism: Escherichia coli strain ST95-32

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: F3J96_RS26685
• Protein ID: WP_000969988.1
• Coordinates: 14775..14957 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: F3J96_RS26680
• Protein ID: WP_001324224.1
• Coordinates: 14581..14778 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3J96_RS26660	9841..10077	+	237	WP_001323890.1	colicin V immunity protein	-
F3J96_RS26665	10055..10366	+	312	WP_001259758.1	colicin V	-
F3J96_RS26670	10536..12650	-	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
F3J96_RS26675	12625..13899	-	1275	WP_000489608.1	HlyD family secretion protein	-
F3J96_RS26680	14581..14778	+	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
F3J96_RS26685	14775..14957	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F3J96_RS26690	15056..15364	+	309	WP_000377483.1	hypothetical protein	-
F3J96_RS26700	15924..16958	+	1035	WP_001190233.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..189994	189994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T139663 WP_000969988.1 NZ_CP043952:14775-14957 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T139663 NZ_CP058771:c3570684-3570382 [Shigella flexneri]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCCGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT139663 WP_001324224.1 NZ_CP043952:14581-14778 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT139663 NZ_CP058771:c3570346-3570005 [Shigella flexneri]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTG
AGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGG
GAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A075MDK3