Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 25701..27324 Replicon plasmid pST95-32-1
Accession NZ_CP043951
Organism Escherichia coli strain ST95-32

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag F3J96_RS25545 Protein ID WP_000088046.1
Coordinates 25701..27026 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag F3J96_RS25550 Protein ID WP_024136327.1
Coordinates 27031..27324 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3J96_RS25515 22371..22670 - 300 WP_001572440.1 hypothetical protein -
F3J96_RS25520 22725..22961 - 237 Protein_25 type I restriction-modification system subunit M N-terminal domain-containing protein -
F3J96_RS25525 23415..23840 - 426 WP_001043843.1 hypothetical protein -
F3J96_RS25530 24094..24909 - 816 WP_001572439.1 HNH endonuclease -
F3J96_RS25535 24922..25332 - 411 WP_000985911.1 hypothetical protein -
F3J96_RS25540 25434..25640 - 207 WP_000134171.1 hypothetical protein -
F3J96_RS25545 25701..27026 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
F3J96_RS25550 27031..27324 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
F3J96_RS25560 27928..28320 - 393 WP_000341066.1 hypothetical protein -
F3J96_RS25565 28693..29034 + 342 WP_001151575.1 hypothetical protein -
F3J96_RS25570 29126..29317 + 192 WP_000398480.1 hypothetical protein -
F3J96_RS25575 29367..29648 + 282 WP_000077926.1 hypothetical protein -
F3J96_RS25580 30211..31452 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / floR / tet(A) / qnrS1 / ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / dfrA14 - 1..228062 228062


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T139662 WP_000088046.1 NZ_CP043951:c27026-25701 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T139662 NZ_CP058771:3173211-3173597 [Shigella flexneri]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAAGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCGGGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT139662 WP_024136327.1 NZ_CP043951:c27324-27031 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT139662 NZ_CP058771:3173587-3173916 [Shigella flexneri]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References