Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2827222..2827719 Replicon chromosome
Accession NZ_CP043950
Organism Escherichia coli strain ST95-32

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag F3J96_RS14365 Protein ID WP_001173343.1
Coordinates 2827222..2827395 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag F3J96_RS14370 Protein ID WP_001312331.1
Coordinates 2827423..2827719 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3J96_RS14355 2823892..2825349 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
F3J96_RS14360 2825586..2827103 + 1518 WP_000003806.1 lysine--tRNA ligase -
F3J96_RS14365 2827222..2827395 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
F3J96_RS14370 2827423..2827719 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
F3J96_RS14375 2827947..2828219 - 273 WP_000405647.1 N-acetyltransferase -
F3J96_RS14380 2828231..2828461 - 231 WP_000373293.1 (4Fe-4S)-binding protein -
F3J96_RS14385 2828642..2830273 + 1632 WP_001216482.1 sensor histidine kinase -
F3J96_RS14390 2830270..2830989 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T139646 WP_001173343.1 NZ_CP043950:c2827395-2827222 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T139646 NZ_CP058762:c5674-5324 [Klebsiella pneumoniae]
ATGTGGGATATCATAACAACCGAGTGCTTTGATGGGTGGTTTCTTGCTCAGAGCGATGCTCTCAGGGAGTCTGTCTATGA
GGCTATGGGTGTACTGGAGAAATTCGGCCCGGCACTAGGTAGACCGTATGTTGATACGCTAAACGATTCTGATTTTGCCA
ATATGAAAGAGTTGCGCATCCAACATCATGGTAATCCTGTCAGAGCATTTTTTGCGTTTGATCCTACGCGCCGCGCCATC
GTCTTGTGTGCCGGTGATAAAACAGGGGCAAACGAAAAGCGCTTCTATAAGGATATGATTAGGCTTGCTGATTCGGAATA
CCGTAAACATCTTGCGAAACTGGAGAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT139646 WP_001312331.1 NZ_CP043950:c2827719-2827423 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT139646 NZ_CP058762:c5321-5022 [Klebsiella pneumoniae]
ATGGCTACTTACAGAGAACTCCTGGCTAAAGAAAGCCCTGAAATGCAGGCTAGGGTAGCGGAGCGGGTTGAAGAAGTCAG
CATTCAAATAGCTCTTAGCATGTTACGTGATGAGCTAAATATTTCACAAACTGAGCTGGCTGCTGTCATGGGTGTTAAAC
AACCTTCTGTTGCCCGTATGGAGCAGGCTGACAATGATCCGAGACTATCAACACTCAAACGTTACGTGAAAGCGTTAGGT
GGAGAATTGAGTTTGGATGTAACACTTCCGACTGGTAAGCGAGTTGCATTTCATATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References