Detailed information of TA system TA139636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2372401..2373989
• Replicon: chromosome
• Accession: NZ_CP043950
• Organism: Escherichia coli strain ST95-32

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: F3J96_RS11945
• Protein ID: WP_001125475.1
• Coordinates: 2372401..2373723 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: F3J96_RS11950
• Protein ID: WP_001332145.1
• Coordinates: 2373723..2373989 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3J96_RS11895	2367646..2367837	-	192	WP_000100899.1	hypothetical protein	-
F3J96_RS11900	2367865..2368266	-	402	WP_001329851.1	helix-turn-helix domain-containing protein	-
F3J96_RS11905	2368376..2368648	+	273	WP_000887447.1	helix-turn-helix domain-containing protein	-
F3J96_RS11910	2368632..2368910	+	279	Protein_2268	toxin YdaT domain-containing protein	-
F3J96_RS27795	2368919..2369164	+	246	Protein_2269	hypothetical protein	-
F3J96_RS27800	2369313..2369510	+	198	Protein_2270	host specificity protein J	-
F3J96_RS11925	2370200..2371215	+	1016	Protein_2271	DUF1983 domain-containing protein	-
F3J96_RS11930	2371218..2371817	+	600	WP_001233195.1	Ail/Lom family outer membrane beta-barrel protein	-
F3J96_RS11935	2371969..2372247	+	279	Protein_2273	prophage tail fiber N-terminal domain-containing protein	-
F3J96_RS11945	2372401..2373723	-	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
F3J96_RS11950	2373723..2373989	-	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
F3J96_RS11955	2374297..2375055	+	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
F3J96_RS11960	2375059..2375973	-	915	WP_001306069.1	bestrophin family protein	-
F3J96_RS11965	2376170..2377621	-	1452	WP_000854637.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T139636 WP_001125475.1 NZ_CP043950:c2373723-2372401 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T139636 NZ_CP058758:1841108-1841210 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT139636 WP_001332145.1 NZ_CP043950:c2373989-2373723 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT139636 NZ_CP058758:c1841217-1841072 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8