Detailed information of TA system TA139624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 838681..839039
• Replicon: chromosome
• Accession: NZ_CP043950
• Organism: Escherichia coli strain ST95-32

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: F3J96_RS27710
• Protein ID: WP_001332330.1
• Coordinates: 838917..839039 (+)
• Length: 41 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: F3J96_RS04235
• Protein ID: WP_000125566.1
• Coordinates: 838681..838914 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3J96_RS04210	833754..834041	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
F3J96_RS04215	834100..835431	+	1332	WP_150330593.1	MFS transporter	-
F3J96_RS04220	835539..836069	+	531	WP_000600749.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
F3J96_RS04225	836062..837924	+	1863	WP_021514787.1	glutathione-regulated potassium-efflux system protein KefC	-
F3J96_RS04230	838116..838595	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
F3J96_RS04235	838681..838914	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
F3J96_RS27710	838917..839039	+	123	WP_001332330.1	CcdB family protein	Toxin
F3J96_RS27715	839080..839232	+	153	WP_146692624.1	CcdB family protein	-
F3J96_RS04245	839229..840077	-	849	WP_000257201.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
F3J96_RS04250	840084..840461	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
F3J96_RS04255	840464..841285	-	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
F3J96_RS04260	841282..842271	-	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
F3J96_RS04265	842271..843557	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-39)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4563.34 Da
• Isoelectric Point: 10.9266

>T139624 WP_001332330.1 NZ_CP043950:838917-839039 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Nucleotide

• Length: 123 bp

>T139624 NZ_CP058752:4257378-4257746 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT139624 WP_000125566.1 NZ_CP043950:838681-838914 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT139624 NZ_CP058752:4257160-4257381 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9