Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 27396..27921 Replicon plasmid pMPNDM-5
Accession NZ_CP043948
Organism Escherichia coli strain AR202.2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag F3P13_RS24350 Protein ID WP_001159871.1
Coordinates 27616..27921 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag F3P13_RS24345 Protein ID WP_000813630.1
Coordinates 27396..27614 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3P13_RS24315 22606..23292 + 687 Protein_22 IS1 family transposase -
F3P13_RS24320 23546..24568 - 1023 WP_000361402.1 helicase UvrD -
F3P13_RS24325 24553..26118 - 1566 WP_001128474.1 AAA family ATPase -
F3P13_RS24330 26193..26609 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
F3P13_RS24335 26606..26836 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
F3P13_RS24345 27396..27614 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F3P13_RS24350 27616..27921 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
F3P13_RS24355 27922..28728 + 807 WP_000016970.1 site-specific integrase -
F3P13_RS24360 29450..30205 + 756 WP_000852146.1 replication initiation protein RepE -
F3P13_RS24365 30793..31959 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD / tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / aac(3)-IIa / mph(A) iucA / iucB / iucC / iucD / iutA 1..99454 99454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T139607 WP_001159871.1 NZ_CP043948:27616-27921 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T139607 NZ_CP058748:c4222290-4222000 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT139607 WP_000813630.1 NZ_CP043948:27396-27614 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT139607 NZ_CP058748:c4222522-4222280 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References