Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4126481..4127057 Replicon chromosome
Accession NZ_CP043946
Organism Escherichia coli strain AR202.2

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag F3P13_RS19920 Protein ID WP_001297643.1
Coordinates 4126770..4127057 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag F3P13_RS19915 Protein ID WP_000063160.1
Coordinates 4126481..4126783 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3P13_RS19900 4123077..4125227 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
F3P13_RS19905 4125277..4125480 + 204 WP_000467859.1 YbdD/YjiX family protein -
F3P13_RS19910 4125491..4126447 + 957 WP_001297640.1 GTPase -
F3P13_RS19915 4126481..4126783 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
F3P13_RS19920 4126770..4127057 - 288 WP_001297643.1 BrnT family toxin Toxin
F3P13_RS19925 4127256..4127952 - 697 Protein_3848 IS1 family transposase -
F3P13_RS19930 4128267..4129499 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
F3P13_RS19935 4129540..4130820 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4127256..4127465 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T139598 WP_001297643.1 NZ_CP043946:c4127057-4126770 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T139598 NZ_CP058748:c2103506-2103399 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT139598 WP_000063160.1 NZ_CP043946:c4126783-4126481 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT139598 NZ_CP058748:2103559-2103620 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References