Detailed information of TA system TA139594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2541575..2542213
• Replicon: chromosome
• Accession: NZ_CP043946
• Organism: Escherichia coli strain AR202.2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: F3P13_RS12290
• Protein ID: WP_001447010.1
• Coordinates: 2542037..2542213 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: F3P13_RS12285
• Protein ID: WP_001270286.1
• Coordinates: 2541575..2541991 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P13_RS12265	2536727..2537668	-	942	WP_001251304.1	ABC transporter permease	-
F3P13_RS12270	2537669..2538682	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
F3P13_RS12275	2538700..2539845	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
F3P13_RS12280	2540090..2541496	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
F3P13_RS12285	2541575..2541991	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
F3P13_RS12290	2542037..2542213	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
F3P13_RS12295	2542435..2542665	+	231	WP_000494244.1	YncJ family protein	-
F3P13_RS12300	2542757..2544718	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
F3P13_RS12305	2544791..2545327	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
F3P13_RS12310	2545380..2546594	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T139594 WP_001447010.1 NZ_CP043946:c2542213-2542037 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T139594 NZ_CP058748:934129-934536 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT139594 WP_001270286.1 NZ_CP043946:c2541991-2541575 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT139594 NZ_CP058748:933882-934148 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8