Detailed information of TA system TA139582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1077380..1077963
• Replicon: chromosome
• Accession: NZ_CP043946
• Organism: Escherichia coli strain AR202.2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: F3P13_RS05270
• Protein ID: WP_000254738.1
• Coordinates: 1077628..1077963 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: F3P13_RS05265
• Protein ID: WP_000581937.1
• Coordinates: 1077380..1077628 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P13_RS05255	1073719..1075020	+	1302	WP_000046812.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
F3P13_RS05260	1075068..1077302	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
F3P13_RS05265	1077380..1077628	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
F3P13_RS05270	1077628..1077963	+	336	WP_000254738.1	endoribonuclease MazF	Toxin
F3P13_RS05275	1078034..1078825	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
F3P13_RS05280	1079053..1080690	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
F3P13_RS05285	1080778..1082076	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T139582 WP_000254738.1 NZ_CP043946:1077628-1077963 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T139582 NZ_CP058743:c4926431-4925514 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT139582 WP_000581937.1 NZ_CP043946:1077380-1077628 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT139582 NZ_CP058743:c4927195-4926437 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTTGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CQY
• PDB: 1MVF
• PDB: 2MRN
• PDB: 2MRU
• AlphaFold DB: A0A7U9LMB4