Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 75891..76416 Replicon plasmid pMPNDM-5
Accession NZ_CP043944
Organism Escherichia coli strain AR216.2b

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag F3P14_RS24700 Protein ID WP_001159871.1
Coordinates 75891..76196 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag F3P14_RS24705 Protein ID WP_000813630.1
Coordinates 76198..76416 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3P14_RS24685 71853..73019 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
F3P14_RS24690 73607..74362 - 756 WP_000852146.1 replication initiation protein RepE -
F3P14_RS24695 75084..75890 - 807 WP_000016970.1 site-specific integrase -
F3P14_RS24700 75891..76196 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
F3P14_RS24705 76198..76416 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F3P14_RS24715 76976..77206 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
F3P14_RS24720 77203..77619 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
F3P14_RS24725 77694..79259 + 1566 WP_001128474.1 AAA family ATPase -
F3P14_RS24730 79244..80266 + 1023 WP_000361402.1 helicase UvrD -
F3P14_RS24735 80520..81206 - 687 Protein_99 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / aac(3)-IIa / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..99465 99465


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T139571 WP_001159871.1 NZ_CP043944:c76196-75891 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T139571 NZ_CP058742:c1863136-1862807 [Streptococcus suis]
ATGGAATTTGAGTCTTATTCAGTGATACTTGCTCCAGCAGTGGAGAAGGAATTGGCAGTTATTTACGCCTATTTTTCGGA
ACAGTTCTCAGAAGAAATTGCCAAAAGACGAATTGGGATGATTGTCGAAGCATTAGAGAGTTTGCAAATTTTTCCTGAAC
GTGGTTTTAACGCCGATAACCGTTTTGGTAAGCAGATAGACCCGCCTCATCTAACCCGAGGCTATGTCGTTGGAAAAGAC
TACATCGCCCTCTATCGCGTAGTGAAAAATGAAGTTCGAGTTGGTCATCTTTTTGCGACCAAGAGTGATTATGTGAAGTT
GTTGAAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT139571 WP_000813630.1 NZ_CP043944:c76416-76198 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT139571 NZ_CP058742:c1863413-1863126 [Streptococcus suis]
TTGATAACTATAAATAAAGATGCCCAAGTAAATTTTAAAACTGATTCCAGACTTTTAGAAAAAGCCAAGGAAGTATTTGC
TATGAACCAGCTGGATTTGACTGCTGGTTTTAACCTCTTCTTGCAAAATATCGCTGTAAAAAATGAGTTGCCAATTCTGA
CAGAAGAAGAGTTGGAAAGAGAAGAGTTGTTTTTACAGTTACAGAAGGAAATCCAGGAAAACCAACAGGCGATTGAGGCA
GGTCACGGGATCAGCCTAGAAAGTGTCAAAGAGAAGTATGGAATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References