Detailed information of TA system TA139559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4127732..4128308
• Replicon: chromosome
• Accession: NZ_CP043942
• Organism: Escherichia coli strain AR216.2b

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: F3P14_RS19930
• Protein ID: WP_001297643.1
• Coordinates: 4128021..4128308 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A080I7H7
• Locus tag: F3P14_RS19925
• Protein ID: WP_000063160.1
• Coordinates: 4127732..4128034 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P14_RS19910	4124328..4126478	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
F3P14_RS19915	4126528..4126731	+	204	WP_000467859.1	YbdD/YjiX family protein	-
F3P14_RS19920	4126742..4127698	+	957	WP_001297640.1	GTPase	-
F3P14_RS19925	4127732..4128034	-	303	WP_000063160.1	BrnA antitoxin family protein	Antitoxin
F3P14_RS19930	4128021..4128308	-	288	WP_001297643.1	BrnT family toxin	Toxin
F3P14_RS19935	4128507..4129203	-	697	Protein_3851	IS1 family transposase	-
F3P14_RS19940	4129518..4130750	+	1233	WP_001137030.1	multidrug efflux MFS transporter MdtM	-
F3P14_RS19945	4130791..4132071	+	1281	WP_001037390.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4128507..4128716	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T139559 WP_001297643.1 NZ_CP043942:c4128308-4128021 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T139559 NZ_CP058740:c1217123-1216278 [Streptococcus suis]
ATGAATAAAGCTAAGCTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGATTAACCTTTAATTCCGTGATGACCTA
TTACTTTCTTGAAGTGATTTTGAAAAAATTAAGTCAGAGTACCTATTCAAATCACTATATATTCAAAGGAGGATTCCTAC
TTTCGAATGTCATCGGAGTTGAATCTCGTAGCACGGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAACAGCAAATTGAAGAAGTTTTAGCAGATTCTGAAAAAGGTATTTCTTTCACAATTCAATCTATCACAGCTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGTGCAACCATTTTGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATTACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTTCAAACCGTCTATTCACGTAACTTCTTGAACAGTAGAAG
CAAAGATTTTTACGATGTTTATATTCTTTCAAAACTGAAAAAAGAAGACATTGACTTCATTCAGTTGAAAAATGCCTGTC
AGAGAACGTTTTCATATCGCGAAACAGAACTTGATTTTGTAAAGATTATTGAACTACTCGAGAGGTTCAAATCTGCCCCT
ATTCAGAATAAACAATGGCAAAATTATTCAAAAAAATATAGCTATACAAAGGGGATTTCACTTGCAGATGTTCTTGATGA
GATGATTAATCTTATTACAATTATTATTTCTACTGACTCTGATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11552.01 Da
• Isoelectric Point: 9.4646

>AT139559 WP_000063160.1 NZ_CP043942:c4128034-4127732 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT139559 NZ_CP043942:c4128034-4127732 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9

Antitoxin

• AlphaFold DB: A0A080I7H7