Detailed information of TA system TA139545

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1920520..1921355
• Replicon: chromosome
• Accession: NZ_CP043942
• Organism: Escherichia coli strain AR216.2b

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: F3P14_RS09255
• Protein ID: WP_042046912.1
• Coordinates: 1920520..1920897 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: F3P14_RS09260
• Protein ID: WP_135145692.1
• Coordinates: 1920987..1921355 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P14_RS09215	1916040..1916513	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
F3P14_RS09220	1916711..1917769	+	1059	WP_001200895.1	FUSC family protein	-
F3P14_RS09225	1917941..1918270	+	330	WP_000450409.1	DUF496 family protein	-
F3P14_RS09230	1918371..1918553	-	183	WP_024198358.1	ethanolamine utilization protein	-
F3P14_RS09240	1919007..1920035	-	1029	Protein_1787	IS110 family transposase	-
F3P14_RS09245	1920194..1920307	-	114	WP_032140621.1	DUF957 domain-containing protein	-
F3P14_RS09250	1920320..1920523	-	204	WP_000761705.1	hypothetical protein	-
F3P14_RS09255	1920520..1920897	-	378	WP_042046912.1	TA system toxin CbtA family protein	Toxin
F3P14_RS09260	1920987..1921355	-	369	WP_135145692.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F3P14_RS09265	1921518..1921739	-	222	WP_000692345.1	DUF987 domain-containing protein	-
F3P14_RS09270	1921802..1921882	-	81	Protein_1793	hypothetical protein	-
F3P14_RS09275	1921969..1922715	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
F3P14_RS09280	1922730..1924271	-	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
F3P14_RS09285	1924412..1924864	-	453	WP_129445215.1	RadC family protein	-
F3P14_RS09290	1924880..1925353	-	474	WP_000855059.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14020.96 Da
• Isoelectric Point: 7.8276

>T139545 WP_042046912.1 NZ_CP043942:c1920897-1920520 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T139545 NZ_CP058725:2130176-2130454 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13545.30 Da
• Isoelectric Point: 6.4764

>AT139545 WP_135145692.1 NZ_CP043942:c1921355-1920987 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPHHQHTVTLYAKGLTCEADTLGSCGYAYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT139545 NZ_CP058725:2129913-2130173 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA