Detailed information of TA system TA139541

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 796315..797147
• Replicon: chromosome
• Accession: NZ_CP043942
• Organism: Escherichia coli strain AR216.2b

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: F3P14_RS03860
• Protein ID: WP_000854765.1
• Coordinates: 796315..796689 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: F3P14_RS03865
• Protein ID: WP_001295723.1
• Coordinates: 796779..797147 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P14_RS03825	791393..791992	+	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-
F3P14_RS03830	791995..792972	+	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
F3P14_RS03835	792969..794147	+	1179	WP_000094991.1	type II secretion system protein GspL	-
F3P14_RS03840	794149..794685	+	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
F3P14_RS03845	794966..795808	-	843	WP_022645115.1	DUF4942 domain-containing protein	-
F3P14_RS03850	795893..796087	-	195	WP_024181941.1	DUF957 domain-containing protein	-
F3P14_RS03855	796112..796315	-	204	WP_001398797.1	hypothetical protein	-
F3P14_RS03860	796315..796689	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
F3P14_RS03865	796779..797147	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F3P14_RS03870	797310..797531	-	222	WP_000692341.1	DUF987 domain-containing protein	-
F3P14_RS03875	797594..798070	-	477	WP_001186774.1	RadC family protein	-
F3P14_RS03880	798086..798565	-	480	WP_057109541.1	antirestriction protein	-
F3P14_RS03885	798831..799649	-	819	WP_001175165.1	DUF945 domain-containing protein	-
F3P14_RS03890	799739..799972	-	234	WP_001278293.1	DUF905 family protein	-
F3P14_RS03895	799978..800655	-	678	WP_001097302.1	hypothetical protein	-
F3P14_RS03900	800803..801483	-	681	WP_001282921.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T139541 WP_000854765.1 NZ_CP043942:c796689-796315 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T139541 NZ_CP058725:c910440-910264 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT139541 WP_001295723.1 NZ_CP043942:c797147-796779 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT139541 NZ_CP058725:c910218-909802 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52