Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 95697..96222 Replicon plasmid pRM14516
Accession NZ_CP043938
Organism Escherichia coli strain RM14516

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FS841_RS24100 Protein ID WP_001159871.1
Coordinates 95697..96002 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag FS841_RS24105 Protein ID WP_000813639.1
Coordinates 96004..96222 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS841_RS24075 (FS841_24380) 91879..92295 - 417 WP_052903861.1 plasmid partitioning/stability family protein -
FS841_RS24080 (FS841_24385) 92288..93268 - 981 WP_000688508.1 plasmid segregation protein ParM -
FS841_RS24085 (FS841_24390) 93683..93979 - 297 WP_052901302.1 hypothetical protein -
FS841_RS24090 (FS841_24395) 94066..94710 - 645 WP_052901305.1 ParA family protein -
FS841_RS24095 (FS841_24400) 94890..95696 - 807 WP_052901307.1 site-specific integrase -
FS841_RS24100 (FS841_24405) 95697..96002 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FS841_RS24105 (FS841_24410) 96004..96222 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FS841_RS24110 (FS841_24415) 96819..97079 + 261 WP_021573941.1 hypothetical protein -
FS841_RS24115 (FS841_24420) 97076..97666 + 591 WP_000194564.1 hypothetical protein -
FS841_RS24120 (FS841_24425) 97684..98031 - 348 WP_052903917.1 DUF6404 family protein -
FS841_RS24125 (FS841_24435) 98516..98836 - 321 Protein_90 colicin-like pore-forming protein -
FS841_RS24130 (FS841_24440) 98946..99677 - 732 WP_052903915.1 conjugal transfer complement resistance protein TraT -
FS841_RS24135 99954..100106 - 153 Protein_92 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..107620 107620


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T139536 WP_001159871.1 NZ_CP043938:c96002-95697 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T139536 NZ_CP058725:241743-241994 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT139536 WP_000813639.1 NZ_CP043938:c96222-96004 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT139536 NZ_CP058725:241991-242245 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References