Detailed information of TA system TA139535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4770992..4771587
• Replicon: chromosome
• Accession: NZ_CP043937
• Organism: Escherichia coli strain RM14516

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: FS841_RS22825
• Protein ID: WP_000239581.1
• Coordinates: 4771237..4771587 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: FS841_RS22820
• Protein ID: WP_001223213.1
• Coordinates: 4770992..4771243 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS841_RS22810 (4766657)	4766657..4770436	+	3780	WP_000060904.1	autotransporter assembly complex protein TamB	-
FS841_RS22815 (4770439)	4770439..4770780	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
FS841_RS22820 (4770992)	4770992..4771243	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
FS841_RS22825 (4771237)	4771237..4771587	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
FS841_RS22830 (4771666)	4771666..4772196	-	531	WP_000055075.1	inorganic diphosphatase	-
FS841_RS22835 (4772506)	4772506..4773462	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
FS841_RS22840 (4773602)	4773602..4775104	+	1503	WP_000205837.1	sugar ABC transporter ATP-binding protein	-
FS841_RS22845 (4775118)	4775118..4776140	+	1023	WP_001367697.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T139535 WP_000239581.1 NZ_CP043937:4771237-4771587 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T139535 NZ_CP058721:19266-19547 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT139535 WP_001223213.1 NZ_CP043937:4770992-4771243 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT139535 NZ_CP058721:19025-19276 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7