Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4616626..4617123 Replicon chromosome
Accession NZ_CP043937
Organism Escherichia coli strain RM14516

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FS841_RS22070 Protein ID WP_001173343.1
Coordinates 4616950..4617123 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FS841_RS22065 Protein ID WP_000398619.1
Coordinates 4616626..4616922 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS841_RS22045 (4613355) 4613355..4614074 - 720 WP_000611288.1 two-component system response regulator DcuR -
FS841_RS22050 (4614071) 4614071..4615702 - 1632 WP_001216477.1 sensor histidine kinase -
FS841_RS22055 (4615883) 4615883..4616113 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
FS841_RS22060 (4616125) 4616125..4616397 + 273 WP_001545144.1 GNAT family N-acetyltransferase -
FS841_RS22065 (4616626) 4616626..4616922 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FS841_RS22070 (4616950) 4616950..4617123 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FS841_RS22075 (4617241) 4617241..4618758 - 1518 WP_000003806.1 lysine--tRNA ligase -
FS841_RS22080 (4618995) 4618995..4620452 - 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T139533 WP_001173343.1 NZ_CP043937:4616950-4617123 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T139533 NZ_CP043937:4616950-4617123 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT139533 WP_000398619.1 NZ_CP043937:4616626-4616922 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT139533 NZ_CP058719:c3881763-3881512 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATAGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References