Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3925719..3926331 Replicon chromosome
Accession NZ_CP043937
Organism Escherichia coli strain RM14516

Toxin (Protein)


Gene name hicA Uniprot ID A0A167AMN3
Locus tag FS841_RS18840 Protein ID WP_000833474.1
Coordinates 3926146..3926331 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1V3VHB3
Locus tag FS841_RS18835 Protein ID WP_000499746.1
Coordinates 3925719..3926129 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS841_RS18820 (3921231) 3921231..3922046 - 816 WP_000891823.1 AraC family transcriptional regulator -
FS841_RS18825 (3922272) 3922272..3923672 + 1401 WP_000204814.1 MFS transporter -
FS841_RS18830 (3923683) 3923683..3925647 + 1965 WP_001545013.1 glycoside hydrolase family 127 protein -
FS841_RS18835 (3925719) 3925719..3926129 - 411 WP_000499746.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FS841_RS18840 (3926146) 3926146..3926331 - 186 WP_000833474.1 type II toxin-antitoxin system HicA family toxin Toxin
FS841_RS18845 (3926600) 3926600..3928138 - 1539 WP_001306609.1 aldehyde dehydrogenase AldB -
FS841_RS18850 (3928339) 3928339..3929490 - 1152 WP_000741501.1 L-threonine dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6819.94 Da        Isoelectric Point: 12.0345

>T139532 WP_000833474.1 NZ_CP043937:c3926331-3926146 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSRHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T139532 NZ_CP058719:c3726388-3726285 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15290.22 Da        Isoelectric Point: 4.5486

>AT139532 WP_000499746.1 NZ_CP043937:c3926129-3925719 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDYAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT139532 NZ_CP058719:3726247-3726518 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAATAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A167AMN3

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3VHB3

References