Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3177940..3178594 Replicon chromosome
Accession NZ_CP043937
Organism Escherichia coli strain RM14516

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag FS841_RS15165 Protein ID WP_000244769.1
Coordinates 3177940..3178347 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FS841_RS15170 Protein ID WP_000354046.1
Coordinates 3178328..3178594 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS841_RS15145 (3173897) 3173897..3175630 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
FS841_RS15150 (3175636) 3175636..3176346 - 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FS841_RS15155 (3176371) 3176371..3177267 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
FS841_RS15160 (3177379) 3177379..3177900 + 522 WP_001055874.1 flavodoxin FldB -
FS841_RS15165 (3177940) 3177940..3178347 - 408 WP_000244769.1 protein YgfX Toxin
FS841_RS15170 (3178328) 3178328..3178594 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FS841_RS15175 (3178837) 3178837..3179817 + 981 WP_000886049.1 tRNA-modifying protein YgfZ -
FS841_RS15180 (3179894) 3179894..3180553 - 660 WP_000250274.1 hemolysin III family protein -
FS841_RS15185 (3180717) 3180717..3181028 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
FS841_RS15190 (3181073) 3181073..3182506 + 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T139529 WP_000244769.1 NZ_CP043937:c3178347-3177940 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T139529 NZ_CP058719:c3082837-3082730 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT139529 WP_000354046.1 NZ_CP043937:c3178594-3178328 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT139529 NZ_CP058719:3082884-3082950 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References