Detailed information of TA system TA139519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1703279..1704867
• Replicon: chromosome
• Accession: NZ_CP043937
• Organism: Escherichia coli strain RM14516

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A2K3TSW7
• Locus tag: FS841_RS08165
• Protein ID: WP_001544563.1
• Coordinates: 1703279..1704601 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FS841_RS08170
• Protein ID: WP_001296726.1
• Coordinates: 1704601..1704867 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS841_RS08125 (1698314)	1698314..1698886	-	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-
FS841_RS08130 (1699037)	1699037..1699189	-	153	WP_001322417.1	amidohydrolase family protein	-
FS841_RS08135 (1699179)	1699179..1699598	-	420	WP_001544559.1	amidohydrolase family protein	-
FS841_RS08140 (1699558)	1699558..1699713	-	156	WP_001322415.1	hypothetical protein	-
FS841_RS08145 (1699816)	1699816..1700763	-	948	WP_000854796.1	aldo/keto reductase	-
FS841_RS08150 (1700837)	1700837..1701526	-	690	WP_097742342.1	type 1 glutamine amidotransferase domain-containing protein	-
FS841_RS08155 (1701535)	1701535..1701843	-	309	WP_001221046.1	hypothetical protein	-
FS841_RS08160 (1701983)	1701983..1702882	+	900	WP_001057776.1	LysR family transcriptional regulator	-
FS841_RS08165 (1703279)	1703279..1704601	-	1323	WP_001544563.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
FS841_RS08170 (1704601)	1704601..1704867	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49416.09 Da
• Isoelectric Point: 8.8753

>T139519 WP_001544563.1 NZ_CP043937:c1704601-1703279 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T139519 NZ_CP058719:c980758-980573 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT139519 WP_001296726.1 NZ_CP043937:c1704867-1704601 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT139519 NZ_CP058719:c980556-980146 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A2K3TSW7