Detailed information of TA system TA139517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1604479..1605117
• Replicon: chromosome
• Accession: NZ_CP043937
• Organism: Escherichia coli strain RM14516

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: FS841_RS07725
• Protein ID: WP_000813797.1
• Coordinates: 1604479..1604655 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FS841_RS07730
• Protein ID: WP_076611057.1
• Coordinates: 1604701..1605117 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS841_RS07705 (1600101)	1600101..1601312	-	1212	WP_001323162.1	BenE family transporter YdcO	-
FS841_RS07710 (1601365)	1601365..1601901	+	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
FS841_RS07715 (1601974)	1601974..1603935	+	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FS841_RS07720 (1604027)	1604027..1604257	-	231	WP_000494244.1	YncJ family protein	-
FS841_RS07725 (1604479)	1604479..1604655	+	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FS841_RS07730 (1604701)	1604701..1605117	+	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FS841_RS07735 (1605196)	1605196..1606602	+	1407	WP_097742392.1	PLP-dependent aminotransferase family protein	-
FS841_RS07740 (1606847)	1606847..1607992	+	1146	WP_097742391.1	ABC transporter substrate-binding protein	-
FS841_RS07745 (1608010)	1608010..1609023	+	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
FS841_RS07750 (1609024)	1609024..1609965	+	942	WP_001251335.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T139517 WP_000813797.1 NZ_CP043937:1604479-1604655 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T139517 NZ_CP043937:1604479-1604655 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT139517 WP_076611057.1 NZ_CP043937:1604701-1605117 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT139517 NZ_CP058719:474459-474794 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829J715