Detailed information of TA system TA139512

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1195177..1196012
• Replicon: chromosome
• Accession: NZ_CP043937
• Organism: Escherichia coli strain RM14516

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: FS841_RS05525
• Protein ID: WP_097742452.1
• Coordinates: 1195635..1196012 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FS841_RS05520
• Protein ID: WP_001598216.1
• Coordinates: 1195177..1195545 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS841_RS05490 (1190783)	1190783..1191769	+	987	WP_097742430.1	protein YcdU	-
FS841_RS05495 (1192099)	1192099..1192513	+	415	Protein_1070	IS200/IS605 family transposase	-
FS841_RS05500 (1192545)	1192545..1193758	+	1214	WP_085949589.1	IS3-like element ISEc31 family transposase	-
FS841_RS05505 (1193841)	1193841..1194320	+	480	WP_097742453.1	antirestriction protein	-
FS841_RS05510 (1194336)	1194336..1194812	+	477	WP_123055190.1	RadC family protein	-
FS841_RS05515 (1194875)	1194875..1195097	+	223	Protein_1074	DUF987 domain-containing protein	-
FS841_RS05520 (1195177)	1195177..1195545	+	369	WP_001598216.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FS841_RS05525 (1195635)	1195635..1196012	+	378	WP_097742452.1	TA system toxin CbtA family protein	Toxin
FS841_RS05530 (1196009)	1196009..1196497	+	489	WP_000761692.1	DUF5983 family protein	-
FS841_RS05535 (1196509)	1196509..1196706	+	198	WP_000839257.1	DUF957 domain-containing protein	-
FS841_RS05540 (1196803)	1196803..1197645	+	843	WP_097742456.1	DUF4942 domain-containing protein	-
FS841_RS05550 (1198116)	1198116..1199054	+	939	WP_097742451.1	glyoxylate/hydroxypyruvate reductase GhrA	-
FS841_RS05555 (1199109)	1199109..1199846	+	738	WP_000283664.1	zinc-binding phosphatase	-
FS841_RS05560 (1199870)	1199870..1200424	+	555	WP_001001917.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgG / csgF / csgE / csgD / csgB / csgA / csgC	1192581..1206582	14001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14189.24 Da
• Isoelectric Point: 8.2905

>T139512 WP_097742452.1 NZ_CP043937:1195635-1196012 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLGRLLDQHYGLTLNDTPFADERVIEQHIEAVISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T139512 NZ_CP058718:c851-570 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13605.40 Da
• Isoelectric Point: 7.3992

>AT139512 WP_001598216.1 NZ_CP043937:1195177-1195545 [Escherichia coli]
VSDTLSGTTHPDDNNSHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSRGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT139512 NZ_CP058718:c1092-841 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA