Detailed information of TA system TA139511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 948427..949132
• Replicon: chromosome
• Accession: NZ_CP043937
• Organism: Escherichia coli strain RM14516

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: FS841_RS04425
• Protein ID: WP_000539521.1
• Coordinates: 948746..949132 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FS841_RS04420
• Protein ID: WP_001280945.1
• Coordinates: 948427..948756 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS841_RS04405 (943584)	943584..944495	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
FS841_RS04410 (944673)	944673..947021	+	2349	WP_001322378.1	EAL domain-containing protein	-
FS841_RS04415 (947029)	947029..948357	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
FS841_RS04420 (948427)	948427..948756	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
FS841_RS04425 (948746)	948746..949132	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FS841_RS04430 (949358)	949358..950683	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
FS841_RS04435 (950896)	950896..951279	+	384	WP_000497137.1	biofilm formation regulator BssR	-
FS841_RS04440 (951390)	951390..952505	+	1116	WP_000555033.1	aldose sugar dehydrogenase YliI	-
FS841_RS04445 (952502)	952502..953128	-	627	WP_001314584.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T139511 WP_000539521.1 NZ_CP043937:c949132-948746 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T139511 NZ_CP058717:77021-77302 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT139511 WP_001280945.1 NZ_CP043937:c948756-948427 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT139511 NZ_CP058717:76780-77031 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6