Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 497717..498335 Replicon chromosome
Accession NZ_CP043937
Organism Escherichia coli strain RM14516

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FS841_RS02290 Protein ID WP_001291435.1
Coordinates 497717..497935 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FS841_RS02295 Protein ID WP_000344800.1
Coordinates 497961..498335 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS841_RS02255 (493006) 493006..493578 + 573 WP_000779833.1 YbaY family lipoprotein -
FS841_RS02260 (493609) 493609..493920 - 312 WP_000409911.1 MGMT family protein -
FS841_RS02270 (494299) 494299..494652 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FS841_RS02275 (494694) 494694..496244 - 1551 WP_001376748.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FS841_RS02280 (496408) 496408..496878 - 471 WP_000136192.1 YlaC family protein -
FS841_RS02285 (496994) 496994..497545 - 552 WP_000102564.1 maltose O-acetyltransferase -
FS841_RS02290 (497717) 497717..497935 - 219 WP_001291435.1 HHA domain-containing protein Toxin
FS841_RS02295 (497961) 497961..498335 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FS841_RS02300 (498881) 498881..502030 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
FS841_RS02305 (502053) 502053..503246 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T139510 WP_001291435.1 NZ_CP043937:c497935-497717 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T139510 NZ_CP058717:28328-28609 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT139510 WP_000344800.1 NZ_CP043937:c498335-497961 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT139510 NZ_CP058717:28087-28338 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References