Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 131068..131711 Replicon plasmid pSCKLB555-3
Accession NZ_CP043935
Organism Klebsiella pneumoniae strain 555

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag F2X40_RS29200 Protein ID WP_001044768.1
Coordinates 131068..131484 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag F2X40_RS29205 Protein ID WP_001261287.1
Coordinates 131481..131711 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F2X40_RS29185 127567..128157 - 591 WP_000194575.1 hypothetical protein -
F2X40_RS29190 128157..128414 - 258 WP_000343085.1 hypothetical protein -
F2X40_RS29195 128768..130906 + 2139 WP_000350635.1 AAA family ATPase -
F2X40_RS29200 131068..131484 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
F2X40_RS29205 131481..131711 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
F2X40_RS29215 132019..133584 + 1566 WP_000741348.1 ATP-binding protein -
F2X40_RS29220 133641..134508 - 868 Protein_159 hypothetical protein -
F2X40_RS29225 134510..135598 - 1089 WP_000952231.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-IIa / oqxB / oqxA / sul2 / tet(A) / floR / blaCTX-M-55 / blaTEM-1B / fosA3 / aph(3'')-Ib / aph(6)-Id - 1..179179 179179


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T139507 WP_001044768.1 NZ_CP043935:c131484-131068 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T139507 NZ_CP058715:15233-15511 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCTTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT139507 WP_001261287.1 NZ_CP043935:c131711-131481 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT139507 NZ_CP043935:c131711-131481 [Klebsiella pneumoniae]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References