Detailed information of TA system TA139498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 21335..21857
• Replicon: plasmid pSCKLB555-3
• Accession: NZ_CP043935
• Organism: Klebsiella pneumoniae strain 555

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: F2X40_RS28455
• Protein ID: WP_000220561.1
• Coordinates: 21576..21857 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: F2X40_RS28450
• Protein ID: WP_000121743.1
• Coordinates: 21335..21586 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F2X40_RS28420	16408..16794	-	387	WP_190294488.1	DNA distortion polypeptide 3	-
F2X40_RS28425	16830..17797	+	968	Protein_21	IS5-like element IS903B family transposase	-
F2X40_RS28430	17963..18407	-	445	Protein_22	hypothetical protein	-
F2X40_RS28435	18411..19247	-	837	Protein_23	replication initiation protein	-
F2X40_RS28440	19268..20102	-	835	Protein_24	replication initiation protein	-
F2X40_RS28450	21335..21586	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
F2X40_RS28455	21576..21857	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F2X40_RS28460	22095..22187	-	93	Protein_27	IS6 family transposase	-
F2X40_RS28465	22353..23558	-	1206	Protein_28	IS91 family transposase	-
F2X40_RS28470	23558..23746	-	189	WP_000957857.1	hypothetical protein	-
F2X40_RS28475	23866..25294	+	1429	Protein_30	IS4-like element IS50R family transposase	-
F2X40_RS28480	25323..26117	+	795	WP_000572405.1	aminoglycoside O-phosphotransferase APH(3')-IIa	-
F2X40_RS28485	26138..26374	+	237	Protein_32	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-IIa / oqxB / oqxA / sul2 / tet(A) / floR / blaCTX-M-55 / blaTEM-1B / fosA3 / aph(3'')-Ib / aph(6)-Id	-	1..179179	179179
	-	inside	IScluster/Tn	aph(3')-IIa / oqxB / oqxA / sul2 / tet(A) / floR	-	15693..50891	35198

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11023.86 Da
• Isoelectric Point: 10.5938

>T139498 WP_000220561.1 NZ_CP043935:21576-21857 [Klebsiella pneumoniae]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T139498 NZ_CP058714:c2652359-2651895 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT139498 WP_000121743.1 NZ_CP043935:21335-21586 [Klebsiella pneumoniae]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT139498 NZ_CP058714:c2652694-2652359 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301