Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 68587..69497 Replicon plasmid pSCKLB555-1
Accession NZ_CP043933
Organism Klebsiella pneumoniae strain 555

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag F2X40_RS26450 Protein ID WP_004181896.1
Coordinates 69027..69497 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag F2X40_RS26445 Protein ID WP_004181895.1
Coordinates 68587..69030 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F2X40_RS26420 64848..65270 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
F2X40_RS26425 65270..66361 + 1092 Protein_80 translesion error-prone DNA polymerase V subunit UmuC -
F2X40_RS26430 66431..67212 - 782 Protein_81 AAA family ATPase -
F2X40_RS26435 67209..68228 - 1020 WP_150102507.1 IS21-like element ISSen3 family transposase -
F2X40_RS26440 68322..68486 + 165 Protein_83 DUF4113 domain-containing protein -
F2X40_RS26445 68587..69030 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
F2X40_RS26450 69027..69497 + 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
F2X40_RS26455 69607..69867 - 261 WP_004026352.1 hypothetical protein -
F2X40_RS26465 70590..70748 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
F2X40_RS30570 71351..72324 + 974 Protein_88 Hok/Gef family protein -
F2X40_RS26475 72522..72899 - 378 WP_004181901.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4 / aph(3'')-Ib / aph(6)-Id - 1..247938 247938


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T139494 WP_004181896.1 NZ_CP043933:69027-69497 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T139494 NZ_CP058714:2232679-2232786 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT139494 WP_004181895.1 NZ_CP043933:68587-69030 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT139494 NZ_CP043933:68587-69030 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References