Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4951098..4951688 Replicon chromosome
Accession NZ_CP043932
Organism Klebsiella pneumoniae strain 555

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag F2X40_RS24315 Protein ID WP_022615588.1
Coordinates 4951356..4951688 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag F2X40_RS24310 Protein ID WP_000288812.1
Coordinates 4951098..4951355 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F2X40_RS24285 4947894..4948862 - 969 WP_102000837.1 IS5 family transposase -
F2X40_RS24290 4948882..4949790 + 909 WP_077268953.1 DUF4917 family protein -
F2X40_RS24295 4950099..4950560 + 462 WP_065808624.1 hypothetical protein -
F2X40_RS24300 4950557..4950763 + 207 WP_065808625.1 helix-turn-helix domain-containing protein -
F2X40_RS24310 4951098..4951355 + 258 WP_000288812.1 antitoxin Antitoxin
F2X40_RS24315 4951356..4951688 + 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F2X40_RS24320 4951781..4952215 - 435 WP_023312731.1 IS200/IS605 family transposase -
F2X40_RS24330 4952640..4954076 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
F2X40_RS24340 4954442..4955896 - 1455 WP_004148975.1 AMP nucleosidase -
F2X40_RS24345 4956026..4956271 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4943449..4952215 8766
- inside IScluster/Tn - - 4947894..4952215 4321


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T139485 WP_022615588.1 NZ_CP043932:4951356-4951688 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T139485 NZ_CP058713:72089-72469 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT139485 WP_000288812.1 NZ_CP043932:4951098-4951355 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT139485 NZ_CP058713:71868-72089 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References