Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3048317..3048942 Replicon chromosome
Accession NZ_CP043932
Organism Klebsiella pneumoniae strain 555

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag F2X40_RS14915 Protein ID WP_019705794.1
Coordinates 3048317..3048700 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag F2X40_RS14920 Protein ID WP_004150355.1
Coordinates 3048700..3048942 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F2X40_RS14900 3045683..3046585 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
F2X40_RS14905 3046582..3047217 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
F2X40_RS14910 3047214..3048143 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
F2X40_RS14915 3048317..3048700 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
F2X40_RS14920 3048700..3048942 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
F2X40_RS14925 3049147..3050064 + 918 WP_004146235.1 alpha/beta hydrolase -
F2X40_RS14930 3050079..3051020 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
F2X40_RS14935 3051065..3051502 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
F2X40_RS14940 3051499..3052359 - 861 WP_004146232.1 virulence factor BrkB family protein -
F2X40_RS14945 3052353..3052952 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T139482 WP_019705794.1 NZ_CP043932:c3048700-3048317 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T139482 NZ_CP058709:3798939-3799274 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT139482 WP_004150355.1 NZ_CP043932:c3048942-3048700 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT139482 NZ_CP058709:3798691-3798939 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References