Detailed information of TA system TA139454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-YefM
• Location: 303005..303576
• Replicon: plasmid pSX1_LC6
• Accession: NZ_CP043903
• Organism: Shewanella sp. LC6

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FJD32_RS24030
• Protein ID: WP_140907296.1
• Coordinates: 303005..303316 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1Z3MW28
• Locus tag: FJD32_RS24035
• Protein ID: WP_011787805.1
• Coordinates: 303316..303576 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJD32_RS24020	298990..299865	-	876	WP_181317832.1	sel1 repeat family protein	-
FJD32_RS24025	299895..302981	-	3087	WP_205403065.1	Tn3 family transposase	-
FJD32_RS24030	303005..303316	-	312	WP_140907296.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FJD32_RS24035	303316..303576	-	261	WP_011787805.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FJD32_RS24040	303841..304305	-	465	WP_140907297.1	cation transporter	-
FJD32_RS24045	304313..304597	-	285	WP_069455589.1	mercury resistance system periplasmic binding protein MerP	-
FJD32_RS24050	304632..304979	-	348	WP_058689194.1	mercury transporter	-
FJD32_RS24055	305057..305458	-	402	WP_069455590.1	Hg(II)-responsive transcriptional regulator	-
FJD32_RS24060	305656..305997	-	342	Protein_312	L-methionine/branched-chain amino acid transporter	-
FJD32_RS24065	306209..306640	+	432	WP_037426668.1	Lrp/AsnC family transcriptional regulator	-
FJD32_RS24070	306909..307850	-	942	WP_047537963.1	cytochrome c nitrite reductase subunit NrfD	-
FJD32_RS24075	307847..308533	-	687	WP_047537960.1	4Fe-4S dicluster domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aadA2 / qacE / sul1	-	1..355688	355688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-61)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11835.67 Da
• Isoelectric Point: 8.5341

>T139454 WP_140907296.1 NZ_CP043903:c303316-303005 [Shewanella sp. LC6]
MPVTYHLTPDAQSDLIGIHRFTLAQWGTTQSKTYLSGLRQTILLLAETPTLGKNRPEVRMNVFSFPYSSHVIYYIQHEHQ
FVVFGILHKSMVPLAHLAERETI

Nucleotide

• Length: 312 bp

>T139454 NZ_CP058682:c5218307-5218134 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9312.71 Da
• Isoelectric Point: 6.4546

>AT139454 WP_011787805.1 NZ_CP043903:c303576-303316 [Shewanella sp. LC6]
MNTLSANEAKIHFGDLLLKAQQAPIQINKNGKPVAVVISADAYQSIETLKLHLLQSKAVKAITDIKMGNLVDGNTFFDEL
AAGQYD

Nucleotide

• Length: 261 bp

>AT139454 NZ_CP058682:c5218631-5218335 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MW28