Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 5022..5593 Replicon plasmid p5
Accession NZ_CP043901
Organism Leptospira interrogans serovar Bataviae strain 1489

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag Lepto1489_RS22005 Protein ID WP_000240418.1
Coordinates 5252..5593 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag Lepto1489_RS22000 Protein ID WP_000443825.1
Coordinates 5022..5258 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Lepto1489_RS21980 536..691 + 156 WP_001976969.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
Lepto1489_RS21985 1257..2255 - 999 WP_192505233.1 Rpn family recombination-promoting nuclease/putative transposase -
Lepto1489_RS21990 2527..3735 - 1209 WP_192505234.1 leucine-rich repeat domain-containing protein -
Lepto1489_RS21995 3945..4159 - 215 Protein_4 hypothetical protein -
Lepto1489_RS22000 5022..5258 + 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
Lepto1489_RS22005 5252..5593 + 342 WP_000240418.1 endoribonuclease MazF Toxin
Lepto1489_RS22010 5957..6190 + 234 WP_000557895.1 hypothetical protein -
Lepto1489_RS22015 6900..7685 + 786 WP_061266477.1 hypothetical protein -
Lepto1489_RS22020 7776..8465 + 690 WP_000850445.1 hypothetical protein -
Lepto1489_RS22025 8513..9361 - 849 WP_000480703.1 IS3 family transposase -
Lepto1489_RS22030 9361..9546 - 186 WP_192505252.1 hypothetical protein -
Lepto1489_RS22035 9516..9650 - 135 WP_192505253.1 transposase -
Lepto1489_RS22040 9700..9876 - 177 WP_000801909.1 hypothetical protein -
Lepto1489_RS22045 10129..10428 + 300 WP_000835630.1 type II toxin-antitoxin system Phd/YefM family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..69692 69692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T139446 WP_000240418.1 NZ_CP043901:5252-5593 [Leptospira interrogans serovar Bataviae]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T139446 NZ_CP058682:c2785901-2785614 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT139446 WP_000443825.1 NZ_CP043901:5022-5258 [Leptospira interrogans serovar Bataviae]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT139446 NZ_CP058682:c2786140-2785901 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References