Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazE-MazF
Location 50321..50886 Replicon plasmid p2
Accession NZ_CP043898
Organism Leptospira interrogans serovar Bataviae strain 1489

Toxin (Protein)


Gene name mazF Uniprot ID A0A0F6H5X2
Locus tag Lepto1489_RS21345 Protein ID WP_000648189.1
Coordinates 50321..50569 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0C5XAD8
Locus tag Lepto1489_RS21350 Protein ID WP_000237066.1
Coordinates 50563..50886 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Lepto1489_RS21315 45337..45717 + 381 WP_000410390.1 hypothetical protein -
Lepto1489_RS21320 45724..46623 - 900 WP_192505209.1 hypothetical protein -
Lepto1489_RS21325 46648..47268 - 621 WP_002105527.1 hypothetical protein -
Lepto1489_RS21330 47513..47713 + 201 WP_192505215.1 hypothetical protein -
Lepto1489_RS21335 49052..49357 + 306 WP_000844596.1 hypothetical protein -
Lepto1489_RS21340 49330..50016 + 687 WP_082271315.1 restriction endonuclease -
Lepto1489_RS21345 50321..50569 + 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Toxin
Lepto1489_RS21350 50563..50886 + 324 WP_000237066.1 type II toxin-antitoxin system PemK/MazF family toxin Antitoxin
Lepto1489_RS21355 51009..51179 + 171 WP_002077237.1 hypothetical protein -
Lepto1489_RS21360 51263..51640 - 378 WP_000603456.1 hypothetical protein -
Lepto1489_RS21365 52254..52394 + 141 WP_002077221.1 hypothetical protein -
Lepto1489_RS21370 52570..53023 + 454 Protein_45 hypothetical protein -
Lepto1489_RS21375 53157..54305 - 1149 Protein_46 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..93306 93306


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

SpoVT-AbrB domain

(6-49)

Antitoxin

mRNA interferase PemK-like

(2-105)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9507.04 Da        Isoelectric Point: 5.1749

>T139443 WP_000648189.1 NZ_CP043898:50321-50569 [Leptospira interrogans serovar Bataviae]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Download         Length: 249 bp

>T139443 NZ_CP043898:50321-50569 [Leptospira interrogans serovar Bataviae]
ATGAAGGCTTCAGTCGTAAAAATAGGCAATTCCAAAGGTATTCGAATTCCCAAGGCAGTCTTAGAAGAATGTCATATTGA
AGAAGAAGTAGATTTACTAATCGATAATAACAAATTAATTATTGTACCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAAACAATGTCATCTAATAAAGATGATAAATTATTTATTCCTGATTCAATAGATTTATTAGACAAGGATTGG
GAATGGTGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12168.59 Da        Isoelectric Point: 10.5161

>AT139443 WP_000237066.1 NZ_CP043898:50563-50886 [Leptospira interrogans serovar Bataviae]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Download         Length: 324 bp

>AT139443 NZ_CP058682:c2484545-2484354 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6H5X2

Antitoxin

Source ID Structure
AlphaFold DB A0A0C5XAD8

References