Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1529272..1529848 Replicon chromosome
Accession NZ_CP043893
Organism Leptospira interrogans serovar Bataviae strain 1489

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag Lepto1489_RS06260 Protein ID WP_000617906.1
Coordinates 1529507..1529848 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag Lepto1489_RS06255 Protein ID WP_000844758.1
Coordinates 1529272..1529520 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Lepto1489_RS06240 1524924..1526906 + 1983 WP_000802799.1 HD family phosphohydrolase -
Lepto1489_RS06245 1527548..1528006 - 459 WP_000453265.1 hypothetical protein -
Lepto1489_RS06250 1528160..1528456 + 297 WP_000477061.1 helix-turn-helix domain-containing protein -
Lepto1489_RS06255 1529272..1529520 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
Lepto1489_RS06260 1529507..1529848 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
Lepto1489_RS06265 1530872..1531864 - 993 WP_000041092.1 aldolase -
Lepto1489_RS06270 1532004..1532939 - 936 WP_000004530.1 ornithine carbamoyltransferase -
Lepto1489_RS06275 1533743..1534210 + 468 WP_000060051.1 hypothetical protein -
Lepto1489_RS06280 1534207..1534611 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T139442 WP_000617906.1 NZ_CP043893:1529507-1529848 [Leptospira interrogans serovar Bataviae]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T139442 NZ_CP058682:c2478865-2478671 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACAGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT139442 WP_000844758.1 NZ_CP043893:1529272-1529520 [Leptospira interrogans serovar Bataviae]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT139442 NZ_CP058682:2478494-2478672 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References