Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 3120..3685 Replicon plasmid p3
Accession NZ_CP043890
Organism Leptospira interrogans serovar Canicola strain 782

Toxin (Protein)


Gene name mazE Uniprot ID A0A0C5XAD8
Locus tag Lepto782_RS22015 Protein ID WP_000237066.1
Coordinates 3120..3443 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazF Uniprot ID A0A0F6H5X2
Locus tag Lepto782_RS22020 Protein ID WP_000648189.1
Coordinates 3437..3685 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Lepto782_RS21995 983..1436 - 454 Protein_1 hypothetical protein -
Lepto782_RS22000 1612..1752 - 141 WP_002077221.1 hypothetical protein -
Lepto782_RS22005 2366..2743 + 378 WP_000603456.1 hypothetical protein -
Lepto782_RS22010 2827..2997 - 171 WP_002077237.1 hypothetical protein -
Lepto782_RS22015 3120..3443 - 324 WP_000237066.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
Lepto782_RS22020 3437..3685 - 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
Lepto782_RS22025 3990..4676 - 687 WP_082271315.1 restriction endonuclease -
Lepto782_RS22030 4649..4954 - 306 WP_000844596.1 hypothetical protein -
Lepto782_RS22035 6115..7413 + 1299 WP_000558495.1 group II intron reverse transcriptase/maturase -
Lepto782_RS22040 7781..8263 - 483 WP_002092119.1 hypothetical protein -
Lepto782_RS22045 8260..8544 - 285 WP_061234873.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..77059 77059
- flank IS/Tn - - 1..849 848


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-105)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12168.59 Da        Isoelectric Point: 10.5161

>T139438 WP_000237066.1 NZ_CP043890:c3443-3120 [Leptospira interrogans serovar Canicola]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Download         Length: 324 bp

>T139438 NZ_CP058682:c2338057-2337950 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9507.04 Da        Isoelectric Point: 5.1749

>AT139438 WP_000648189.1 NZ_CP043890:c3685-3437 [Leptospira interrogans serovar Canicola]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Download         Length: 249 bp

>AT139438 NZ_CP058682:2338107-2338170 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C5XAD8

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6H5X2

References