Detailed information of TA system TA139437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 37089..37660
• Replicon: plasmid p4
• Accession: NZ_CP043888
• Organism: Leptospira interrogans serovar Canicola strain 782

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q8F5A3
• Locus tag: Lepto782_RS21405
• Protein ID: WP_000240418.1
• Coordinates: 37319..37660 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A868AD61
• Locus tag: Lepto782_RS21400
• Protein ID: WP_061226369.1
• Coordinates: 37089..37325 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Lepto782_RS21380	32399..33301	-	903	WP_001089277.1	hypothetical protein	-
Lepto782_RS21385	33317..33943	-	627	WP_000078444.1	hypothetical protein	-
Lepto782_RS21390	34884..35360	+	477	WP_000691822.1	hypothetical protein	-
Lepto782_RS21395	35742..36749	+	1008	WP_000628975.1	hypothetical protein	-
Lepto782_RS21400	37089..37325	+	237	WP_061226369.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Lepto782_RS21405	37319..37660	+	342	WP_000240418.1	endoribonuclease MazF	Toxin
Lepto782_RS21410	37728..38111	-	384	WP_000617796.1	hypothetical protein	-
Lepto782_RS21415	38111..38377	-	267	WP_000844450.1	hypothetical protein	-
Lepto782_RS21420	38446..39495	+	1050	WP_061250713.1	IS481 family transposase	-
Lepto782_RS21425	39693..40154	+	462	WP_061234540.1	hypothetical protein	-
Lepto782_RS21430	40285..40872	+	588	WP_061234538.1	hypothetical protein	-
Lepto782_RS21435	41550..42452	-	903	WP_149767132.1	Rpn family recombination-promoting nuclease/putative transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74118	74118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12918.12 Da
• Isoelectric Point: 9.9595

>T139437 WP_000240418.1 NZ_CP043888:37319-37660 [Leptospira interrogans serovar Canicola]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T139437 NZ_CP058682:c2042772-2042395 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCTCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAACAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8710.92 Da
• Isoelectric Point: 5.6663

>AT139437 WP_061226369.1 NZ_CP043888:37089-37325 [Leptospira interrogans serovar Canicola]
MESIIQKWGNSLGIRIPKAIATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT139437 NZ_CP058682:c2043230-2042862 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A4D8SL30