Detailed information of TA system TA139436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 3494405..3495033
• Replicon: chromosome
• Accession: NZ_CP043884
• Organism: Leptospira interrogans serovar Canicola strain 782

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8F7E4
• Locus tag: Lepto782_RS14855
• Protein ID: WP_000281042.1
• Coordinates: 3494635..3495033 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A868A7V8
• Locus tag: Lepto782_RS14850
• Protein ID: WP_001192479.1
• Coordinates: 3494405..3494635 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Lepto782_RS14825	3490540..3491124	+	585	WP_061234864.1	Eco29kI family restriction endonuclease	-
Lepto782_RS14830	3491330..3492253	+	924	WP_061234327.1	M28 family peptidase	-
Lepto782_RS14835	3492478..3493167	+	690	WP_000590462.1	SH3 domain-containing protein	-
Lepto782_RS14840	3493452..3493898	+	447	WP_000869702.1	putative toxin-antitoxin system toxin component, PIN family	-
Lepto782_RS14845	3493882..3494145	+	264	WP_061234326.1	toxin-antitoxin system HicB family antitoxin	-
Lepto782_RS14850	3494405..3494635	+	231	WP_001192479.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Lepto782_RS14855	3494635..3495033	+	399	WP_000281042.1	type II toxin-antitoxin system VapC family toxin	Toxin
Lepto782_RS14860	3495288..3495581	+	294	WP_061234323.1	BrnT family toxin	-
Lepto782_RS14865	3495568..3495840	+	273	WP_061234322.1	BrnA antitoxin family protein	-
Lepto782_RS14870	3495939..3496493	+	555	WP_192505381.1	hypothetical protein	-
Lepto782_RS14880	3497868..3498944	+	1077	WP_192505383.1	hypothetical protein	-
Lepto782_RS14885	3498945..3499403	+	459	WP_001239009.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15139.76 Da
• Isoelectric Point: 9.6893

>T139436 WP_000281042.1 NZ_CP043884:3494635-3495033 [Leptospira interrogans serovar Canicola]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Nucleotide

• Length: 399 bp

>T139436 NZ_CP058682:2029713-2029967 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8741.03 Da
• Isoelectric Point: 8.6588

>AT139436 WP_001192479.1 NZ_CP043884:3494405-3494635 [Leptospira interrogans serovar Canicola]
MQTAKLFINGRSQAVRLPKEFQFAGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Nucleotide

• Length: 231 bp

>AT139436 NZ_CP058682:2029465-2029716 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0F6HAN6