Detailed information of TA system TA139435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/PemK(toxin)
• Location: 3003297..3003873
• Replicon: chromosome
• Accession: NZ_CP043884
• Organism: Leptospira interrogans serovar Canicola strain 782

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: -
• Locus tag: Lepto782_RS12740
• Protein ID: WP_192505335.1
• Coordinates: 3003297..3003638 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: Q93MT9
• Locus tag: Lepto782_RS12745
• Protein ID: WP_000844758.1
• Coordinates: 3003625..3003873 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Lepto782_RS12720	2998664..2999068	-	405	WP_001974506.1	pyridoxamine 5'-phosphate oxidase family protein	-
Lepto782_RS12725	2999065..2999532	-	468	WP_000060053.1	hypothetical protein	-
Lepto782_RS12730	3000336..3001271	+	936	WP_000004530.1	ornithine carbamoyltransferase	-
Lepto782_RS12735	3001411..3002403	+	993	WP_000041092.1	aldolase	-
Lepto782_RS12740	3003297..3003638	-	342	WP_192505335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Lepto782_RS12745	3003625..3003873	-	249	WP_000844758.1	ribbon-helix-helix domain-containing protein	Antitoxin
Lepto782_RS12750	3004689..3004985	-	297	WP_000477061.1	helix-turn-helix domain-containing protein	-
Lepto782_RS12755	3005139..3005598	+	460	Protein_2519	hypothetical protein	-
Lepto782_RS12760	3006240..3008222	-	1983	WP_000802799.1	HD family phosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (2-41)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12371.30 Da
• Isoelectric Point: 9.7995

>T139435 WP_192505335.1 NZ_CP043884:c3003638-3003297 [Leptospira interrogans serovar Canicola]
MIRGKIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Nucleotide

• Length: 342 bp

>T139435 NZ_CP058682:2029465-2029716 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9380.77 Da
• Isoelectric Point: 10.3815

>AT139435 WP_000844758.1 NZ_CP043884:c3003873-3003625 [Leptospira interrogans serovar Canicola]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Nucleotide

• Length: 249 bp

>AT139435 NZ_CP058682:2029713-2029967 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0F6HD92