Detailed information of TA system TA139434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 43682..44256
• Replicon: plasmid p3
• Accession: NZ_CP043883
• Organism: Leptospira interrogans serovar Bataviae strain 1548

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: Lepto1548_RS20100
• Protein ID: WP_000240417.1
• Coordinates: 43912..44256 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: Lepto1548_RS20095
• Protein ID: WP_000443825.1
• Coordinates: 43682..43918 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Lepto1548_RS20065	38807..39424	-	618	WP_000579473.1	hypothetical protein	-
Lepto1548_RS20070	39629..40170	+	542	Protein_30	TPM domain-containing protein	-
Lepto1548_RS20075	40174..40352	+	179	Protein_31	MmcQ/YjbR family DNA-binding protein	-
Lepto1548_RS20080	40652..41704	-	1053	WP_002130468.1	DUF1565 domain-containing protein	-
Lepto1548_RS20085	42663..42875	+	213	WP_000447737.1	hypothetical protein	-
Lepto1548_RS20090	42924..43346	+	423	WP_000402980.1	hypothetical protein	-
Lepto1548_RS20095	43682..43918	+	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Lepto1548_RS20100	43912..44256	+	345	WP_000240417.1	endoribonuclease MazF	Toxin
Lepto1548_RS20110	45556..47400	-	1845	WP_000203569.1	ATP-binding protein	-
Lepto1548_RS20115	47613..49076	+	1464	WP_000888145.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..51265	51265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12984.27 Da
• Isoelectric Point: 9.9595

>T139434 WP_000240417.1 NZ_CP043883:43912-44256 [Leptospira interrogans serovar Bataviae]
MVKNRIYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLILT

Nucleotide

• Length: 345 bp

>T139434 NZ_CP058682:1385409-1385807 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT139434 WP_000443825.1 NZ_CP043883:43682-43918 [Leptospira interrogans serovar Bataviae]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT139434 NZ_CP058682:1385182-1385409 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0F6HC33