Detailed information of TA system TA139433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 3298323..3298951
• Replicon: chromosome
• Accession: NZ_CP043880
• Organism: Leptospira interrogans serovar Bataviae strain 1548

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8F7E4
• Locus tag: Lepto1548_RS13770
• Protein ID: WP_000281042.1
• Coordinates: 3298553..3298951 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A868A7V8
• Locus tag: Lepto1548_RS13765
• Protein ID: WP_001192479.1
• Coordinates: 3298323..3298553 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Lepto1548_RS13745	3293621..3294670	-	1050	WP_001257771.1	hypothetical protein	-
Lepto1548_RS13750	3294820..3295509	+	690	WP_000590461.1	SH3 domain-containing protein	-
Lepto1548_RS13755	3295781..3296083	+	303	WP_001092687.1	hypothetical protein	-
Lepto1548_RS13760	3296129..3297967	+	1839	WP_001015573.1	hypothetical protein	-
Lepto1548_RS13765	3298323..3298553	+	231	WP_001192479.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Lepto1548_RS13770	3298553..3298951	+	399	WP_000281042.1	type II toxin-antitoxin system VapC family toxin	Toxin
Lepto1548_RS13775	3299049..3299360	+	312	WP_000479561.1	hypothetical protein	-
Lepto1548_RS13780	3299371..3299598	+	228	WP_000130601.1	hypothetical protein	-
Lepto1548_RS13785	3299740..3300820	+	1081	Protein_2721	hypothetical protein	-
Lepto1548_RS13790	3300821..3301279	+	459	WP_001239009.1	GNAT family N-acetyltransferase	-
Lepto1548_RS13795	3301495..3302355	-	861	WP_000682239.1	hypothetical protein	-
Lepto1548_RS13800	3302866..3303624	-	759	WP_000636956.1	DUF1554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15139.76 Da
• Isoelectric Point: 9.6893

>T139433 WP_000281042.1 NZ_CP043880:3298553-3298951 [Leptospira interrogans serovar Bataviae]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Nucleotide

• Length: 399 bp

>T139433 NZ_CP058682:1108119-1108430 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8741.03 Da
• Isoelectric Point: 8.6588

>AT139433 WP_001192479.1 NZ_CP043880:3298323-3298553 [Leptospira interrogans serovar Bataviae]
MQTAKLFINGRSQAVRLPKEFQFAGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Nucleotide

• Length: 231 bp

>AT139433 NZ_CP058682:1108427-1108846 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F6HAN6