Detailed information of TA system TA139431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 27879..28450
• Replicon: plasmid p1
• Accession: NZ_CP043878
• Organism: Leptospira interrogans serovar Icterohaemorrhagiae strain Langkawi

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q8F5A3
• Locus tag: LeptoLang_RS19595
• Protein ID: WP_000240418.1
• Coordinates: 28109..28450 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: LeptoLang_RS19590
• Protein ID: WP_000443825.1
• Coordinates: 27879..28115 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LeptoLang_RS19570	24407..25420	+	1014	WP_000427241.1	hypothetical protein	-
LeptoLang_RS19575	25641..25796	+	156	WP_001976969.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
LeptoLang_RS19580	26850..27062	+	213	WP_000447737.1	hypothetical protein	-
LeptoLang_RS19585	27111..27534	+	424	Protein_17	hypothetical protein	-
LeptoLang_RS19590	27879..28115	+	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LeptoLang_RS19595	28109..28450	+	342	WP_000240418.1	endoribonuclease MazF	Toxin
LeptoLang_RS19600	28518..28901	-	384	WP_000617796.1	hypothetical protein	-
LeptoLang_RS19605	28901..29167	-	267	WP_000844450.1	hypothetical protein	-
LeptoLang_RS19610	29228..29569	-	342	WP_000727289.1	hypothetical protein	-
LeptoLang_RS19615	29572..30297	-	726	WP_000414903.1	hypothetical protein	-
LeptoLang_RS19620	30260..30406	-	147	WP_167493406.1	hypothetical protein	-
LeptoLang_RS19625	30555..30710	+	156	Protein_25	BrnT family toxin	-
LeptoLang_RS19630	30722..30922	+	201	WP_001206464.1	hypothetical protein	-
LeptoLang_RS19635	31445..31906	+	462	WP_000811791.1	hypothetical protein	-
LeptoLang_RS19640	32013..32624	+	612	WP_000851077.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63414	63414

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12918.12 Da
• Isoelectric Point: 9.9595

>T139431 WP_000240418.1 NZ_CP043878:28109-28450 [Leptospira interrogans serovar Icterohaemorrhagiae]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T139431 NZ_CP058682:871331-871738 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT139431 WP_000443825.1 NZ_CP043878:27879-28115 [Leptospira interrogans serovar Icterohaemorrhagiae]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT139431 NZ_CP058682:871084-871350 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCTGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A4D8SL30

Antitoxin

• AlphaFold DB: A0A0F6HC33