Detailed information of TA system TA139430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 3281595..3282223
• Replicon: chromosome
• Accession: NZ_CP043876
• Organism: Leptospira interrogans serovar Icterohaemorrhagiae strain Langkawi

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8F7E4
• Locus tag: LeptoLang_RS13520
• Protein ID: WP_000281042.1
• Coordinates: 3281825..3282223 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q8F7E3
• Locus tag: LeptoLang_RS13515
• Protein ID: WP_001192482.1
• Coordinates: 3281595..3281825 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LeptoLang_RS13490	3277425..3277634	-	210	WP_000041838.1	hypothetical protein	-
LeptoLang_RS13495	3277766..3278395	+	630	WP_001081629.1	helix-turn-helix domain-containing protein	-
LeptoLang_RS13500	3278602..3279525	+	924	WP_000680711.1	M28 family peptidase	-
LeptoLang_RS13505	3279749..3280438	+	690	WP_000590463.1	SH3 domain-containing protein	-
LeptoLang_RS13510	3280670..3281347	+	678	WP_000071998.1	hypothetical protein	-
LeptoLang_RS13515	3281595..3281825	+	231	WP_001192482.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LeptoLang_RS13520	3281825..3282223	+	399	WP_000281042.1	type II toxin-antitoxin system VapC family toxin	Toxin
LeptoLang_RS13525	3282321..3282869	+	549	WP_000479567.1	hypothetical protein	-
LeptoLang_RS13530	3283012..3284088	+	1077	WP_001170567.1	hypothetical protein	-
LeptoLang_RS13535	3284089..3284547	+	459	WP_001239009.1	GNAT family N-acetyltransferase	-
LeptoLang_RS13540	3284764..3285624	-	861	WP_000682233.1	hypothetical protein	-
LeptoLang_RS13545	3286165..3286932	-	768	WP_000636952.1	DUF1554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15139.76 Da
• Isoelectric Point: 9.6893

>T139430 WP_000281042.1 NZ_CP043876:3281825-3282223 [Leptospira interrogans serovar Icterohaemorrhagiae]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Nucleotide

• Length: 399 bp

>T139430 NZ_CP058682:c611383-610919 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8771.05 Da
• Isoelectric Point: 8.6588

>AT139430 WP_001192482.1 NZ_CP043876:3281595-3281825 [Leptospira interrogans serovar Icterohaemorrhagiae]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Nucleotide

• Length: 231 bp

>AT139430 NZ_CP058682:c611718-611383 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
ACCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0F6HAN6

Antitoxin

• AlphaFold DB: A0A0F6HAF7