Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1509864..1510440 Replicon chromosome
Accession NZ_CP043876
Organism Leptospira interrogans serovar Icterohaemorrhagiae strain Langkawi

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag LeptoLang_RS06240 Protein ID WP_000617906.1
Coordinates 1510099..1510440 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag LeptoLang_RS06235 Protein ID WP_000844758.1
Coordinates 1509864..1510112 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LeptoLang_RS06220 1505515..1507497 + 1983 WP_000836895.1 HD family phosphohydrolase -
LeptoLang_RS06225 1508139..1508597 - 459 WP_000453266.1 hypothetical protein -
LeptoLang_RS06230 1508751..1509047 + 297 WP_000477061.1 helix-turn-helix domain-containing protein -
LeptoLang_RS06235 1509864..1510112 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
LeptoLang_RS06240 1510099..1510440 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LeptoLang_RS06245 1511349..1512341 - 993 WP_000041092.1 aldolase -
LeptoLang_RS06250 1512481..1513416 - 936 WP_000004530.1 ornithine carbamoyltransferase -
LeptoLang_RS06255 1514220..1514687 + 468 WP_000060051.1 hypothetical protein -
LeptoLang_RS06260 1514684..1515088 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T139429 WP_000617906.1 NZ_CP043876:1510099-1510440 [Leptospira interrogans serovar Icterohaemorrhagiae]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T139429 NZ_CP058673:35879-36184 [Salmonella enterica]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGTGATGAAAGCTGGCGCATGATGACCTCCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAACGCCATTAACCTGATGTTTTGGGGAATATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT139429 WP_000844758.1 NZ_CP043876:1509864-1510112 [Leptospira interrogans serovar Icterohaemorrhagiae]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT139429 NZ_CP058673:35659-35877 [Salmonella enterica]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References