Detailed information of TA system TA139419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 227094..228717
• Replicon: plasmid unnamed2
• Accession: NZ_CP043855
• Organism: Enterobacter hormaechei strain EB_P6_L3_02.19

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: F1867_RS25815
• Protein ID: WP_000088044.1
• Coordinates: 227392..228717 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: F1867_RS25810
• Protein ID: WP_012477377.1
• Coordinates: 227094..227387 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1867_RS25780	222893..223285	+	393	WP_000341066.1	hypothetical protein	-
F1867_RS25790	223944..224600	+	657	WP_000462754.1	trypsin-like serine protease	-
F1867_RS25800	224803..225300	+	498	WP_000340139.1	membrane protein	-
F1867_RS25805	225305..226693	+	1389	WP_000083579.1	hypothetical protein	-
F1867_RS25810	227094..227387	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
F1867_RS25815	227392..228717	+	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
F1867_RS25820	228778..228984	+	207	WP_000134171.1	hypothetical protein	-
F1867_RS25825	229085..229495	+	411	WP_000985909.1	hypothetical protein	-
F1867_RS25830	229508..230323	+	816	WP_001641519.1	HNH endonuclease	-
F1867_RS25835	230576..231001	+	426	WP_000278471.1	hypothetical protein	-
F1867_RS25840	231550..231858	+	309	WP_001224686.1	hypothetical protein	-
F1867_RS25845	231874..232731	+	858	WP_000784387.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / qnrB1	-	1..270269	270269

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T139419 WP_000088044.1 NZ_CP043855:227392-228717 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T139419 NZ_CP058671:2582543-2582761 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT139419 WP_012477377.1 NZ_CP043855:227094-227387 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT139419 NZ_CP058671:2582141-2582515 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7