Detailed information of TA system TA139398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4355282..4355858
• Replicon: chromosome
• Accession: NZ_CP043852
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: F1719_RS21340
• Protein ID: WP_001297643.1
• Coordinates: 4355282..4355569 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: F1719_RS21345
• Protein ID: WP_000063156.1
• Coordinates: 4355556..4355858 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1719_RS21320	4351535..4352815	-	1281	WP_001037382.1	DUF445 domain-containing protein	-
F1719_RS21325	4352856..4354088	-	1233	WP_001339512.1	multidrug efflux MFS transporter MdtM	-
F1719_RS21330	4354234..4354329	-	96	Protein_4041	dienelactone hydrolase family protein	-
F1719_RS21335	4354386..4355083	+	698	Protein_4042	IS1 family transposase	-
F1719_RS21340	4355282..4355569	+	288	WP_001297643.1	BrnT family toxin	Toxin
F1719_RS21345	4355556..4355858	+	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
F1719_RS21350	4355892..4356848	-	957	WP_001297640.1	GTPase	-
F1719_RS21355	4356859..4357062	-	204	WP_000467859.1	YbdD/YjiX family protein	-
F1719_RS21360	4357112..4359262	-	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4354874..4355083	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T139398 WP_001297643.1 NZ_CP043852:4355282-4355569 [Escherichia coli ATCC 8739]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T139398 NZ_CP058663:c106789-106631 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT139398 WP_000063156.1 NZ_CP043852:4355556-4355858 [Escherichia coli ATCC 8739]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT139398 NZ_CP058663:c106864-106833 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: A0A829GEV9

Antitoxin

• AlphaFold DB: A0A829G8Z0