Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4121991..4122488 Replicon chromosome
Accession NZ_CP043852
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag F1719_RS20235 Protein ID WP_001173343.1
Coordinates 4122315..4122488 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag F1719_RS20230 Protein ID WP_000398619.1
Coordinates 4121991..4122287 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1719_RS20210 4118722..4119441 - 720 WP_000611288.1 two-component system response regulator DcuR -
F1719_RS20215 4119438..4121069 - 1632 WP_001216477.1 sensor histidine kinase -
F1719_RS20220 4121250..4121480 + 231 WP_001070602.1 (4Fe-4S)-binding protein -
F1719_RS20225 4121492..4121764 + 273 WP_000405651.1 N-acetyltransferase -
F1719_RS20230 4121991..4122287 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
F1719_RS20235 4122315..4122488 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
F1719_RS20240 4122607..4124124 - 1518 WP_001295090.1 lysine--tRNA ligase -
F1719_RS20245 4124361..4125818 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T139397 WP_001173343.1 NZ_CP043852:4122315-4122488 [Escherichia coli ATCC 8739]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T139397 NZ_CP058663:c66770-66564 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT139397 WP_000398619.1 NZ_CP043852:4121991-4122287 [Escherichia coli ATCC 8739]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT139397 NZ_CP058663:66757-66818 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References