Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 72999..73617 Replicon chromosome
Accession NZ_CP043852
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag F1719_RS00945 Protein ID WP_001291435.1
Coordinates 72999..73217 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag F1719_RS00950 Protein ID WP_000344800.1
Coordinates 73243..73617 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1719_RS00910 68288..68860 + 573 WP_000779826.1 lipoprotein -
F1719_RS00915 68891..69202 - 312 WP_000409911.1 MGMT family protein -
F1719_RS00925 69581..69934 + 354 WP_000878140.1 DUF1428 domain-containing protein -
F1719_RS00930 69976..71526 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
F1719_RS00935 71690..72160 - 471 WP_000136192.1 YlaC family protein -
F1719_RS00940 72276..72827 - 552 WP_000102564.1 maltose O-acetyltransferase -
F1719_RS00945 72999..73217 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
F1719_RS00950 73243..73617 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
F1719_RS00955 74163..77312 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
F1719_RS00960 77335..78528 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T139377 WP_001291435.1 NZ_CP043852:c73217-72999 [Escherichia coli ATCC 8739]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T139377 NZ_CP058661:1238508-1238681 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT139377 WP_000344800.1 NZ_CP043852:c73617-73243 [Escherichia coli ATCC 8739]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT139377 NZ_CP058661:1238184-1238480 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References