Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 38688..39525 Replicon chromosome
Accession NZ_CP043852
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag F1719_RS00775 Protein ID WP_000227786.1
Coordinates 38688..39230 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag F1719_RS00780 Protein ID WP_001297137.1
Coordinates 39214..39525 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1719_RS00755 34218..35129 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
F1719_RS00760 35297..35788 + 492 WP_001138904.1 nucleotide binding protein YajQ -
F1719_RS00765 35916..37280 - 1365 WP_001000978.1 MFS transporter -
F1719_RS00770 37697..38632 + 936 WP_001297127.1 sel1 repeat family protein -
F1719_RS00775 38688..39230 - 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
F1719_RS00780 39214..39525 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
F1719_RS00785 39710..40600 - 891 WP_000971336.1 heme o synthase -
F1719_RS00790 40612..40941 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
F1719_RS00795 40941..41555 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
F1719_RS00800 41545..43536 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
F1719_RS00805 43558..44505 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T139376 WP_000227786.1 NZ_CP043852:c39230-38688 [Escherichia coli ATCC 8739]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T139376 NZ_CP058661:c550977-550870 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT139376 WP_001297137.1 NZ_CP043852:c39525-39214 [Escherichia coli ATCC 8739]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT139376 NZ_CP058661:551025-551091 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References