Detailed information of TA system TA139370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 19603..20228
• Replicon: plasmid unnamed
• Accession: NZ_CP043851
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F1719_RS00130
• Protein ID: WP_000911313.1
• Coordinates: 19603..20001 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: F1719_RS00135
• Protein ID: WP_000450520.1
• Coordinates: 20001..20228 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1719_RS00105	14901..15566	+	666	Protein_20	type IV secretion system DNA-binding domain-containing protein	-
F1719_RS00110	15596..17209	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
F1719_RS00115	17240..17590	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
F1719_RS00120	17587..18012	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
F1719_RS00125	18095..19594	+	1500	Protein_24	type IV conjugative transfer system coupling protein TraD	-
F1719_RS00130	19603..20001	-	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
F1719_RS00135	20001..20228	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
F1719_RS00140	20310..23724	+	3415	Protein_27	conjugative relaxase	-
F1719_RS00145	23744..24490	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..116228	116228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T139370 WP_000911313.1 NZ_CP043851:c20001-19603 [Escherichia coli ATCC 8739]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T139370 NZ_CP058658:c99025-98720 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT139370 WP_000450520.1 NZ_CP043851:c20228-20001 [Escherichia coli ATCC 8739]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT139370 NZ_CP058658:c99245-99027 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2