Detailed information of TA system TA139356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 766149..766678
• Replicon: chromosome
• Accession: NZ_CP043843
• Organism: Staphylococcus aureus strain NCCP 16830

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: F1612_RS03785
• Protein ID: WP_000621175.1
• Coordinates: 766316..766678 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: F1612_RS03780
• Protein ID: WP_000948331.1
• Coordinates: 766149..766319 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1612_RS03750	761185..761745	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
F1612_RS03755	761954..762433	+	480	WP_001287078.1	hypothetical protein	-
F1612_RS03760	762426..764009	+	1584	WP_045173493.1	PH domain-containing protein	-
F1612_RS03765	763996..764487	+	492	WP_078103494.1	PH domain-containing protein	-
F1612_RS03770	764491..764850	+	360	WP_000581200.1	holo-ACP synthase	-
F1612_RS03775	764916..766064	+	1149	WP_001281145.1	alanine racemase	-
F1612_RS03780	766149..766319	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
F1612_RS03785	766316..766678	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F1612_RS03790	767028..768029	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
F1612_RS03795	768148..768474	+	327	WP_001052491.1	anti-sigma factor antagonist	-
F1612_RS03800	768476..768955	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
F1612_RS03805	768930..769700	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T139356 WP_000621175.1 NZ_CP043843:766316-766678 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T139356 NZ_CP058656:768143-768439 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT139356 WP_000948331.1 NZ_CP043843:766149-766319 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT139356 NZ_CP043843:766149-766319 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1